ʻAʻohe mea hoʻohui koko ʻohi ʻula
ʻO ka wehewehe pōkole:
No ka ʻike biochemical, hoʻokolohua immunological, serology, etc.
Hoʻopau maikaʻi ka hoʻohana ʻana i ke koko kū hoʻokahi i ka pilikia o ka hoʻopili ʻana i ke koko a kau ʻia ma ka paia, e hōʻoiaʻiʻo ana i ke kūlana kumu o ke koko i ka nui loa a me ka pololei o nā hopena hōʻike.
Ka hope: ʻO ka poʻe me ka hyperglycemia, ʻoi aku ka poʻe e hāpai ana, hilinaʻi i nā ana glucose koko pololei no ka hōʻoia pololei ʻana o ka maʻi diabetes a me ka nānā ʻana.ʻO ka Glycolysis ma hope o ke huki koko, akā naʻe, hoʻemi ka glucose i loko o ke koko i hōʻiliʻili ʻia ma ka lumi wela me ka loaʻa ʻole o kahi stabilizer.ʻO nā mahana anuanu (4 ° C) ke kāohi i ka glycolysis;akā, paʻakikī ka hoʻomaʻalili ʻana a me ka hoʻomaʻamaʻa ʻana i kēlā me kēia koko koko i ke anuanu ma ka hana lapaʻau maʻamau.No laila, hoʻohana ʻia nā preservatives e hoʻopaʻa i ka glucose i ka wā o ka hōʻiliʻili ʻana i ke koko a me nā kaʻina hana i hana ʻia ma ka lumi wela.Ua nānā kēia haʻawina i ka hopena o nā anticoagulants like ʻole (EDTA, heparin, oxalate), me ka ʻole o ka glycolysis inhibitors (NaF, citrate), i ka paʻa o ka glucose i loko o nā ʻāpana plasma - loaʻa mai ke koko i hōʻiliʻili ʻia a mālama ʻia ma ka lumi wela a hiki i 24h.
Nā Hana: Ua hōʻiliʻili ʻia ke koko Venous mai 60 mau mea manawaleʻa;ua māhele ʻia kēlā me kēia mea hāʻawi koko i ʻeono mau paipu, aia kēlā me kēia me kahi hui anti-glycolysis-anticoagulant ʻokoʻa.ʻO Terumo VENOSAFE™ Glycemia paipu i loaʻa iā NaF/citrate buffer)/Na2EDTA;NaF/Na-heparin;a me NaF/K2oxalate.Aia nā paipu Sarstedt i ka NaF/citrate;NaF/Na2EDTA;a me K2EDTA.Ma ka hola 0, 2, 8 a me 24, ua loaʻa ka plasma no nā ana glucose me ka hoʻohana ʻana i nā ʻano Glucose Hexokinase a me Glucose Oxidase, a me ka ADVIA® 1800 Clinical Chemistry System.
Nā hualoaʻa: Ua hōʻike ʻia nā ʻano hana ʻelua i ka glycolysis liʻiliʻi e 24 h (<3.8%) no nā paipu Terumo VENOSAFE™ Glycemia ʻekolu, a me ka pahu Sarstedt S-Monovette GlucoEXACT i loaʻa iā NaF/citrate.ʻOi aku ka kiʻekiʻe o Glycolysis i nā paipu i loaʻa iā NaF/Na2EDTA-wale (11.7%) a me K2EDTA-wale (85%).
Nā hopena: ʻO nā paipu Glycemia (loaʻa iā NaF/citrate buffer/Na2EDTA; NaF/Na-heparin; a me NaF/K2oxalate) a me nā paipu Sarstedt S-Monovette® GlucoEXACT (loaʻa iā NaF/citrate) kūpono no ka hoʻouna ʻana i nā ʻāpana koko holoʻokoʻa i ka hoʻāʻo. hale hana i loko o 24 h ma ka lumi wela.
