Hoʻoulu ka plasma waiwai nui o Platelet i ka angiogenesis i nā ʻiole e hoʻoikaika i ka ulu lauoho

Platelet-rich plasma (PRP) ʻo ia ka manaʻo autologous o nā platelets kanaka i loko o ka plasma.Ma o ka degranulation o ka alpha granules i loko o nā platelets, hiki i ka PRP ke hūnā i nā mea ulu like ʻole, me ka platelet-derived growth factor (PDGF), vascular endothelial growth factor (VEGF), fibroblast growth factor (FGF), hepatocyte growth factor (HGF), a me ka hoʻololi ʻana. mea ulu (TGF), i kākau ʻia e hoʻomaka i ka hoʻōla ʻana i ka ʻeha a hāpai i ka hoʻonui ʻana a me ka hoʻololi ʻana o nā cell endothelial a me nā pericytes i loko o nā endothelial sprouts.

Ua hōʻike ʻia nā kuleana o PRP no ka mālama ʻana i ka ulu ʻana o ka lauoho i nā noiʻi hou.Uebel et al.Ua ʻike ʻo ia e hoʻonui ʻia nā mea ulu o ka platelet plasma i ka hua o nā ʻāpana follicular i ke kaʻina ʻōhule kāne.Ua hōʻike ʻia nā hana hou e hoʻonui ka PRP i ka hoʻonui ʻana o nā pūnaewele papilla dermal a hoʻoulu i ka hoʻololi ʻana o telogen-to-anagen me ka hoʻohana ʻana i nā hiʻohiʻona in vivo a in vitro.Ua hōʻike ʻia kahi haʻawina ʻē aʻe e hoʻolalelale ʻo PRP i ka hoʻoponopono hou ʻana o ka follicle lauoho a hoʻopōkole nui i ka manawa o ka hoʻokumu ʻana o ka lauoho.

Loaʻa ka PRP a me ka platelet-poor plasma (PPP) i ka hoʻopiha piha o nā protein coagulation.Ma ka haʻawina i kēia manawa, ua noiʻi ʻia ka mana o PRP a me PPP i ka ulu ʻana o ka lauoho ma C57BL / 6 mice.ʻO ke kuhiakau he hopena maikaʻi ka PRP i ka ulu ʻana o ka lōʻihi o ka lauoho a hoʻonui i ka nui o nā follicles lauoho.

Holoholona hoʻokolohua

Loaʻa ka 50 olakino C57BL / 6 kāne ʻiole (6 mau wiki, 20 ± 2 g) mai ka Center of Laboratory Animals, Hangzhou Normal University (Hangzhou, Kina).Hānai ʻia nā holoholona i ka meaʻai like a mālama ʻia i kahi kaiapuni mau ma lalo o ka pōʻaiapuni māmā-ʻeleʻele 12:12-h.Ma hope o 1 pule o ka acclimatization, ua hoʻokaʻawale ʻia nā ʻiole i ʻekolu pūʻulu: pūʻulu PRP (n = 10), pūʻulu PPP (n = 10), a pūʻulu mana (n = 10).

Ua ʻae ʻia ka protocol study e ke kōmike ethics institutional o ka noiʻi holoholona ma lalo o ke Law of Animal Research and Statutory Regulations ma Kina.

Ana lōʻihi lauoho

Ma 8, 13, a me 18 mau lā ma hope o ka hoʻopaʻa hope ʻana, ua koho ʻia nā lauoho 10 i kēlā me kēia ʻiole ma ka wahi i koho ʻia.Ua lawe ʻia nā ana lōʻihi o ka lauoho i ʻekolu mau kahua me ka microscope electron, a ua hōʻike ʻia ko lākou awelika e like me nā millimeters.Hoʻokuʻu ʻia nā lauoho elongated a ʻeha paha.

Hematoxylin a me eosin (HE) staining

Hoʻopau ʻia nā laʻana o ka ʻili hope ma 18 mau lā ma hope o ke kolu o ka hoʻokomo ʻana.A laila ua hoʻopaʻa ʻia nā laʻana i 10% neutral buffered formalin, hoʻokomo ʻia i loko o ka paraffin, a ʻoki ʻia i 4 μm.Hoʻomoʻa ʻia nā ʻāpana no 4 h no ka deparaffinization ma 65 ° C, hoʻokomo ʻia i loko o ka ethanol gradient, a laila hoʻopaʻa ʻia me ka hematoxylin no 5 min.Ma hope o ka hoʻokaʻawale ʻana i ka waiʻona waika hydrochloric 1%, ua hoʻokomo ʻia nā ʻāpana i loko o ka wai amonia, hoʻopaʻa ʻia me ka eosin, a holoi ʻia me ka wai i hoʻomaʻemaʻe ʻia.ʻO ka hope, ua hoʻomaʻemaʻe ʻia nā ʻāpana me ka ethanol gradient, hoʻomaʻemaʻe ʻia me ka xylene, kau ʻia me ka resin neutral, a nānā ʻia me ka microscopy māmā (Olympus, Tokyo, Iapana).